Situated on a hill behind the town of Tivoli, next to the massif of Lucretili Mountains of which it almost represents a southern spur, Monte Catillo Nature Reserve is entirely situated in the Municipality of Tivoli and dominates the course of the river Aniene where the Roman Countryside gives way to the first mountain spurs culminating in the Lucretili and Prenestini mountains, and farther on in the Simbruini mountains, in the central part of Lazio.