It is advisable to reach Monticello, housing the Visitor Center of the Nature Reserve, from where most of the itineraries leave. If you come from Florence, take the exit "Valdarno" from A1 and take SP 11 "Lungo Arno" in direction Arezzo; once you reach loc. Acqua Borra, before the bridge over the river Arno, turn left following the road signs to the Visitor Center.
If you come from Arezzo, take SR 69 in direction Montevarchi and, once you reach Levane, turn right in direction Terranuova Bracciolini (SP 11); after crossing the bridge over Arno, it is necessary to turn right and follow the road signs to the Visitor Center. Alternatively, always from Arezzo, you can reach Pian di Chena, taking SP 1 "Setteponti" in direction Castiglion Fibocchi, going beyond Laterina, and following the road signs to Montalto; after Romito Bridge, take the road on the right up to loc. Pian di Chena, from where, near the restaurant, you can take some paths to visit the Reserve.
If you prefer reaching the Nature Reserve by public means of transport, you can use the interurban bus lines of the company Lazzi and Fabbri, connecting Arezzo with Montevarchi along SP 1 "Setteponti" (via Castiglion Fibocchi-Laterina), using the stop in loc. Ponte Romito. By train, the nearest railway station is in Montalto (Laterina Scalo). SITA bus lines connect Arezzo with Florence along SR 69 and have a stop in Montalto.